Assessment of the Immunoprotective Efficacy of Recombinant 14-3-3 Protein and Dense Granule Protein 10 (GRA10) as Candidate Antigens for Rabbit Vaccines against Eimeria intestinalis

Eimeria intestinalis infects rabbits, causing severe intestinal coccidiosis. Prolonged anticoccidial drug use might lead to coccidia resistance and residues in food. Thus, vaccines are required control rabbit In this study, recombinant E. 14-3-3 GRA10 proteins (rEi-14-3-3 rEi-GRA10) were obtained via prokaryotic expression used as subunit vaccines. Fifty 30-day-old rabbits randomly grouped follows: PBS-uninfected group, PBS-infected Trx-His-S rEi-14-3-3 rEi-GRA10 immunized groups. The subcutaneously twice at 2-week intervals, challenged with 7 × 104 sporulated oocysts, sacrificed 14 days later. protective effects assessed clinical signs, relative weight gain, oocyst reduction, mean lesion score, ACI (anticoccidial index), cytokine, specific antibody levels sera. groups had higher gain rates of 81.94% 73.61% (p < 0.05), reduction 86.13% 84.87% respectively. two fewer lesions 0.05) IgG 0.05). Higher IL-2, IL-4, IFN-γ cytokines the group a level observed. values 168.24 159.91, good moderate effects, Both induced humoral immunity rabbits. addition, Th1- Th2-type immune responses. against infection showing better effect.